Rr27^tm1Wrk
Targeted Allele Detail

Summary

• Symbol: Rr27^tm1Wrk
• Name: regulatory region 27; targeted mutation 1, Wolf Reik
• MGI ID: MGI:3050788
• Synonyms: H19^tm1Wrk
• Gene: Rr27 Location: Chr7:142133885-142136283 bp Genetic Position: Chr7, Syntenic
• Alliance: Rr27^tm1Wrk page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:91713
• Parent Cell Line: Other (see notes) (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Modified regulatory region)
• Mutation: Insertion
• Mutation details: A targeting construct containing three copies of a GAL4-binding motif (UAS) and a floxed ura3 and neomycin resistance gene cassette was inserted into the DMR downstream of the CTCF sites. Transient Cre expression excised the ura3 and neo cassette. The knock in did not interfere with non-coding RNA H19 expression. (J:91713)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Rr27 Mutation: 7 strains or lines available

Notes

ES cell line = 293 HEK.

References

• Original: J:91713 Murrell A, et al., Interaction between differentially methylated regions partitions the imprinted genes Igf2 and H19 into parent-specific chromatin loops. Nat Genet. 2004 Aug;36(8):889-93
• All: 1 reference(s)