Cd274^tm1Shr
Targeted Allele Detail

Summary

• Symbol: Cd274^tm1Shr
• Name: CD274 antigen; targeted mutation 1, Arlene H Sharpe
• MGI ID: MGI:3050803
• Synonyms: PD-L1^-
• Gene: Cd274 Location: Chr19:29344855-29365495 bp, + strand Genetic Position: Chr19, 23.88 cM, cytoband C2
• Alliance: Cd274^tm1Shr page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:91480
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: C57BL/6

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: The signal and all of the IgV exon was replaced with a neomycin resistance gene. Southern blot confirmed recombination. Flow cytometry showed lack of expression in B cells, T cells, macrophages or DCs in mutants. (J:91480)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Cd274 Mutation: 70 strains or lines available

References

• Original: J:91480 Latchman YE, et al., PD-L1-deficient mice show that PD-L1 on T cells, antigen-presenting cells, and host tissues negatively regulates T cells. Proc Natl Acad Sci U S A. 2004 Jul 20;101(29):10691-6
• All: 40 reference(s)