Fhl5^tm1Saco
Targeted Allele Detail

Summary

• Symbol: Fhl5^tm1Saco
• Name: four and a half LIM domains 5; targeted mutation 1, Paolo Sassone-Corsi
• MGI ID: MGI:3050807
• Synonyms: Act^-
• Gene: Fhl5 Location: Chr4:25199907-25242852 bp, - strand Genetic Position: Chr4, 10.58 cM, cytoband A3
• Alliance: Fhl5^tm1Saco page

Testis histology of Fhl5^tm1Saco/Fhl5^tm1Saco and +/+ wild-type mice.

Show the 3 phenotype image(s) involving this allele.

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:91483
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129S2/SvPas

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Exon 2, containing the ATG, was replaced with a PGK-neo cassette via homologous recombination. Southern blot was used to confirm correct insertion of the vector. RNase protection assay showed lack of transcript in mutant testis. Western blot indicated absence of protein in testis extracts from mutants. (J:91483)

Expression

In Mice Carrying this Mutation:	1 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	3 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Fhl5 Mutation: 16 strains or lines available

References

• Original: J:91483 Kotaja N, et al., Abnormal sperm in mice with targeted deletion of the act (activator of cAMP-responsive element modulator in testis) gene. Proc Natl Acad Sci U S A. 2004 Jul 20;101(29):10620-5
• All: 2 reference(s)