Lmnb1^Gt(XA130)Byg
Gene trapped Allele Detail

Summary

• Symbol: Lmnb1^Gt(XA130)Byg
• Name: lamin B1; gene trap XA130, BayGenomics
• MGI ID: MGI:3050819
• Synonyms: Lmnb1^delta, Lmnb1^{Gt(Betageo)1Reue}, Lmnb1^{Gt(pGTopfs)1Ucd}
• Gene: Lmnb1 Location: Chr18:56840885-56886496 bp, + strand Genetic Position: Chr18, 30.84 cM
• Alliance: Lmnb1^Gt(XA130)Byg page

Appearance of Lmnb1^Gt(XA130)Byg/Lmnb1^Gt(XA130)Byg embryos

Show the 2 phenotype image(s) involving this allele.

Mutation origin

• Mutant Cell Line: XA130
• Germline Transmission: Earliest citation of germline transmission: J:91473
• Parent Cell Line: Other (see notes) (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Gene trapped (Null/knockout, Reporter)
• Mutation: Insertion of gene trap vector Vector: pGT0pfs Vector Type: gene trap
• Mutation details: A gene trap containing a Beta-geo reporter gene was inserted into intron 5, resulting in a fusion transcript containing the endogenous amino-terminal head domain and a truncated alpha-helical central rod domain fused to Beta-geo. The protein lacked the endogenous carboxyl-terminal 273 amino acids of Lmb1, thereby eliminating one of two known phosphorylation sites, the nuclear localization signal, and the carboxyl-terminal CAAX motif. Only the 5' sequences were detected in mutant embryos by RT-PCR and Western blot showed no protein. (J:91473)
• Sequence Tags: Sequence tag details (1 tag)

  Tag ID	GenBank ID	Method	Tag Location (trapped strand)*	Select
  XA130	CC144515 ; (MGI Seq Detail)	5' RACE	Chr18:56866299-56866406 bp (+)
  * GRCm39; (strand of gene trap mutagenesis)

• Genome Context: Genome Browser view of this mutation;

  38 gene trap insertions have trapped this gene; View all gene trap sequence tags in this region

Expression

In Mice Carrying this Mutation:	18 assay results
In Structures Affected by this Mutation:	8 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Lmnb1 Mutation: 39 strains or lines available

Notes

All BayGenomics gene trap mutations were generated in either CGR8 or E14TG2a (129P2/OlaHsd) parental ES cell lines, with the majority in subline E14TG2a.4. The specific ES cell line in which each mutation was made is not specified.

References

• Original: J:91473 Vergnes L, et al., Lamin B1 is required for mouse development and nuclear integrity. Proc Natl Acad Sci U S A. 2004 Jul 13;101(28):10428-33
• All: 8 reference(s)