Unc13a^tm2Bros
Targeted Allele Detail

Summary

• Symbol: Unc13a^tm2Bros
• Name: unc-13 homolog A; targeted mutation 2, Nils Brose
• MGI ID: MGI:3051217
• Synonyms: Munc13-1^H567K
• Gene: Unc13a Location: Chr8:72079356-72124418 bp, - strand Genetic Position: Chr8, 34.43 cM, cytoband B3.3
• Alliance: Unc13a^tm2Bros page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:74347
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Hypomorph)
• Mutation: Nucleotide substitutions
• Mutation details: A knock-in strategy was used to insert an H567K point mutation. Crossing mice with EIIa-cre mice resulted in the removal of a floxed neo downstream of the exon containing the mutation. Southern blot and PCR confirmed recombination. Western blot indicated protein levels similar to that of wild-type. (J:74347)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Unc13a Mutation: 74 strains or lines available

References

• Original: J:74347 Rhee JS, et al., Beta phorbol ester- and diacylglycerol-induced augmentation of transmitter release is mediated by Munc13s and not by PKCs. Cell. 2002 Jan 11;108(1):121-33
• All: 6 reference(s)