Rr27^tm2Wrk
Targeted Allele Detail

Summary

• Symbol: Rr27^tm2Wrk
• Name: regulatory region 27; targeted mutation 2, Wolf Reik
• MGI ID: MGI:3051218
• Synonyms: H19^deltaK, H19^tm2Wrk
• Gene: Rr27 Location: Chr7:142133885-142136283 bp Genetic Position: Chr7, Syntenic
• Alliance: Rr27^tm2Wrk page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:91593
• Parent Cell Line: E14 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Modified regulatory region, Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A 1.7 kb direct tandem repeat unit in the DMR (the K repeat) was replaced with a floxed neomycin resistance gene cassette, which was subsequently excised via transiently expressed Cre-mediated recombination. Northern blot showed no significant difference in gene expression between mutant and wild-type tissues. (J:91593)

Expression

In Mice Carrying this Mutation:

36 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Rr27 Mutation: 7 strains or lines available

References

• Original: J:91593 Lewis A, et al., Tandem repeat hypothesis in imprinting: deletion of a conserved direct repeat element upstream of H19 has no effect on imprinting in the Igf2-H19 region. Mol Cell Biol. 2004 Jul;24(13):5650-6
• All: 1 reference(s)