Cat^tm1Ysh
Targeted Allele Detail

Summary

• Symbol: Cat^tm1Ysh
• Name: catalase; targeted mutation 1, Ye-Shih Ho
• MGI ID: MGI:3051232
• Synonyms: Cat^-, catalase^-
• Gene: Cat Location: Chr2:103284249-103315498 bp, - strand Genetic Position: Chr2, 54.43 cM
• Alliance: Cat^tm1Ysh page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:91752
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Part of intron 4 and exon 5 were replaced by a neo cassette. Southern blot confirmed recombination. A 50-70% decrease in mRNA was found in heterozygotes and no mRNA was found in homozygotes by Northern blot analysis. Western blot indicated absence of protein in mutant tissue samples. (J:91752)

Expression

In Mice Carrying this Mutation:

1 RNA-Seq or microarray experiment(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Cat Mutation: 30 strains or lines available

References

• Original: J:91752 Ho YS, et al., Mice lacking catalase develop normally but show differential sensitivity to oxidant tissue injury. J Biol Chem. 2004 Jul 30;279(31):32804-12
• All: 10 reference(s)