Atp2b1^tm1Ges
Targeted Allele Detail

Summary

• Symbol: Atp2b1^tm1Ges
• Name: ATPase, Ca++ transporting, plasma membrane 1; targeted mutation 1, Gary E Shull
• MGI ID: MGI:3051541
• Synonyms: Pmca1^-
• Gene: Atp2b1 Location: Chr10:98750268-98862005 bp, + strand Genetic Position: Chr10, 50.97 cM, cytoband C3
• Alliance: Atp2b1^tm1Ges page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:91844
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129X1/SvJ

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Codons 466-529 in exon 10, including the catalytic phosphorylation site (aspartic acid 475), were replaced with a neomycin resistance gene. Northern blot indicated no difference in levels of mRNA between wild-type and heterozygous mice. RT-PCR confirmed that the exon 9 was spliced to exon 11, producing the transcript of the expected length. (J:91844)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Atp2b1 Mutation: 66 strains or lines available

References

• Original: J:91844 Okunade GW, et al., Targeted ablation of plasma membrane Ca2+-ATPase (PMCA) 1 and 4 indicates a major housekeeping function for PMCA1 and a critical role in hyperactivated sperm motility and male fertility for PMCA4. J Biol Chem. 2004 Aug 6;279(32):33742-50
• All: 4 reference(s)