Atp2b4^tm1Ges
Targeted Allele Detail

Summary

• Symbol: Atp2b4^tm1Ges
• Name: ATPase, Ca++ transporting, plasma membrane 4; targeted mutation 1, Gary E Shull
• MGI ID: MGI:3051542
• Synonyms: Pmca4^-
• Gene: Atp2b4 Location: Chr1:133630416-133728755 bp, - strand Genetic Position: Chr1, 57.95 cM
• Alliance: Atp2b4^tm1Ges page

Atp2b4^tm1Ges/Atp2b4^tm1Ges portal vein smooth muscle shows an increase in apoptosis

Show the 2 phenotype image(s) involving this allele.

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:91844
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129X1/SvJ

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A region of exon 11 encoding amino acids 448-474, including the catalytic phosphorylation site (aspartic acid 465), was replaced with a neomycin resistance gene. Northern blot showed no reduction in mRNA levels in mutant mice. Exon-11-deficient mice express a mutant PMCA4 mRNA as a consequence of exon-10 splicing to exon-12, which preserves the reading frame and enables these mice to produce functionless mutant PMCA4a and PMCA4b proteins (devoid of a catalytic site). (J:91844, J:210683)

Expression

In Structures Affected by this Mutation:

3 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Atp2b4 Mutation: 55 strains or lines available

References

• Original: J:91844 Okunade GW, et al., Targeted ablation of plasma membrane Ca2+-ATPase (PMCA) 1 and 4 indicates a major housekeeping function for PMCA1 and a critical role in hyperactivated sperm motility and male fertility for PMCA4. J Biol Chem. 2004 Aug 6;279(32):33742-50
• All: 14 reference(s)