Ghrh^tm1Salv
Targeted Allele Detail

Summary

• Symbol: Ghrh^tm1Salv
• Name: growth hormone releasing hormone; targeted mutation 1, Roberto Salvatori
• MGI ID: MGI:3051561
• Synonyms: GHRH-, GHRHKO
• Gene: Ghrh Location: Chr2:157171417-157189426 bp, - strand Genetic Position: Chr2, 78.25 cM
• Alliance: Ghrh^tm1Salv page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:91828
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129/Sv

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Part of intron 2, the last 3 amino acids of the N-terminal signal peptide and the first 14 amino acids of the mature protein, in exon 3, were replaced by a neomycin resistance gene. Southern blotting confirmed recombination in ES cells and in homozygous mice. RT-PCR indicated lack of transcript in mutant hypothalamus, testis and placenta. mRNA content in mutant pituitary was significantly less than that in wild-types and heterozygotes and Western blot confirmed this by showing a reduction in the intensity of protein in mutant pituitary and no protein in testes. (J:91828)

Disease models

Expression

In Mice Carrying this Mutation:	1 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	4 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Ghrh Mutation: 14 strains or lines available

References

• Original: J:91828 Alba M, et al., A mouse with targeted ablation of the growth hormone-releasing hormone gene: a new model of isolated growth hormone deficiency. Endocrinology. 2004 Sep;145(9):4134-43
• All: 14 reference(s)