Gja5^tm1(Gja7)Hvr
Targeted Allele Detail

Summary

• Symbol: Gja5^tm1(Gja7)Hvr
• Name: gap junction protein, alpha 5; targeted mutation 1, Harold van Rijen
• MGI ID: MGI:3051866
• Synonyms: Cx40KI45, Cx40^KICx45
• Gene: Gja5 Location: Chr3:96939718-96960950 bp, + strand Genetic Position: Chr3, 42.04 cM
• Alliance: Gja5^tm1(Gja7)Hvr page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:92018
• Parent Cell Line: CK35 (ES Cell)
• Strain of Origin: 129S2/SvPas

Mutation description

• Allele Type: Targeted (Inserted expressed sequence, Null/knockout)
• Mutation: Insertion
• Mutation details: A targeting vector containing exon 3 (the coding exon) of Gjc1 (Gja7), a floxed neo, and exon 2 (the coding exon) of Gja5 with a loxP site near the 3' end was inserted immediately after the start site of the endogenous gene, within exon 2, so as to allow for the expression of Gjc1 in place of Gja5. The second amino acid of Gjc1 was mutated from a serine to a glycine. Mice transmitting the mutation through the germ line were crossed with either CAG-Cre or Cre-deleter mice to excise the floxed neo and most of exon 2 of Gja5. Southern blot and PCR confirmed recombination. RT-PCR, Northern blot and Western blot indicated exon 3 from Gjc1 was expressed in knock-in mice, while Gja5 was not expressed. (J:92018)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gja5 Mutation: 19 strains or lines available

References

• Original: J:92018 Alcolea S, et al., Replacement of connexin40 by connexin45 in the mouse: impact on cardiac electrical conduction. Circ Res. 2004 Jan 9;94(1):100-9
• All: 5 reference(s)