Cckar^tm1Kpn
Targeted Allele Detail

Summary

• Symbol: Cckar^tm1Kpn
• Name: cholecystokinin A receptor; targeted mutation 1, Alan S Kopin
• MGI ID: MGI:3051882
• Synonyms: CCK-1R^-, CCK1R-, CCK-AR^-
• Gene: Cckar Location: Chr5:53855827-53865046 bp, - strand Genetic Position: Chr5, 29.52 cM
• Alliance: Cckar^tm1Kpn page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:53711
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A PGK-neo replaced exon 3, which encodes a portion of the third transmembrane domain and the second intracellular loop including the "ERY" motif. Southern blot of mutant mice indicated successful recombination. Testing for 125I-CCK-8 binding in pancreatic membranes mutant mice indicated showed no displaceable binding and therefore a lack a Cckar receptors. (J:53711)

Expression

In Mice Carrying this Mutation:	1 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	4 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Cckar Mutation: 41 strains or lines available

References

• Original: J:53711 Kopin AS, et al., The cholecystokinin-A receptor mediates inhibition of food intake yet is not essential for the maintenance of body weight [published erratum appears in J Clin Invest 1999 Mar;103(5):759]. J Clin Invest. 1999 Feb;103(3):383-91
• All: 21 reference(s)