Pik3cg^tm1Ehi
Targeted Allele Detail

Summary

• Symbol: Pik3cg^tm1Ehi
• Name: phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit gamma; targeted mutation 1, Emilio Hirsch
• MGI ID: MGI:3051886
• Synonyms: PI3Kgamma^KD
• Gene: Pik3cg Location: Chr12:32223472-32258658 bp, - strand Genetic Position: Chr12, 13.74 cM, cytoband B
• Alliance: Pik3cg^tm1Ehi page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:91945
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Humanized sequence)
• Mutations: Insertion, Nucleotide substitutions
• Mutation details: Exon 2 was replaced by a mutated form of human cDNA, in which the Lys833 was converted into Arg, followed by a neomycin resistance gene (Neo^r) cassette flanked by loxP sequences. Heterozygous mice were crossed with Balancer cre mice to delete the Neo^r cassette. RT-PCR revealed exclusive production of mutant mRNA. (J:91945)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Pik3cg Mutation: 59 strains or lines available

References

• Original: J:91945 Patrucco E, et al., PI3Kgamma modulates the cardiac response to chronic pressure overload by distinct kinase-dependent and -independent effects. Cell. 2004 Aug 6;118(3):375-87
• All: 41 reference(s)