Dapk1^tm1Yuk
Targeted Allele Detail

Summary

• Symbol: Dapk1^tm1Yuk
• Name: death associated protein kinase 1; targeted mutation 1, Kazunori Yukawa
• MGI ID: MGI:3052030
• Synonyms: DAPK
• Gene: Dapk1 Location: Chr13:60749761-60911005 bp, + strand Genetic Position: Chr13, 32.53 cM
• Alliance: Dapk1^tm1Yuk page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:92102
• Parent Cell Line: E14.1 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Insertion of a neomycin resistance gene deleted part of the exon encoding amino acids 22-95 in the catalytic kinase domain. The replaced region encodes a conserved lysine residue that is critical for the kinase activity of the gene. Western blot analysis demonstrated the existence of the mutant protein with a slightly smaller size that wild-type. RT-PCR analysis of kidneys identified the alternative transcript. (J:92102)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Dapk1 Mutation: 126 strains or lines available

References

• Original: J:92102 Yukawa K, et al., Deletion of the kinase domain in death-associated protein kinase attenuates renal tubular cell apoptosis in chronic obstructive uropathy. Int J Mol Med. 2004 Apr;13(4):515-20
• All: 5 reference(s)