Crb1^tm1Wij
Targeted Allele Detail

Summary

• Symbol: Crb1^tm1Wij
• Name: crumbs family member 1, photoreceptor morphogenesis associated; targeted mutation 1, Jan Wijnholds
• MGI ID: MGI:3052072
• Synonyms: Crb1^-, Crb1^ex1
• Gene: Crb1 Location: Chr1:139124794-139304838 bp, - strand Genetic Position: Chr1, 60.87 cM
• Alliance: Crb1^tm1Wij page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:92068
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Modified isoform(s))
• Mutations: Insertion, Intragenic deletion
• Mutation details: A hygromycin resistance cassette replaced 2.9 kb of the upstream promoter region, exon 1 and part of intron 1. Southern blot confirmed a smaller product size and immunoprecipitation indicated no detectable protein. However, LrCaptureSeq data indicates that deletion of this region disrupts isoforms A, A2, and C but leaves isoform B intact. (J:92068, J:293375)

Disease models

Expression

In Mice Carrying this Mutation:	1 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	5 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Crb1 Mutation: 92 strains or lines available

References

• Original: J:92068 Van De Pavert SA, et al., Crumbs homologue 1 is required for maintenance of photoreceptor cell polarization and adhesion during light exposure. J Cell Sci. 2004 Aug 15;117(Pt 18):4169-77
• All: 15 reference(s)