Myh10^tm3.1Rsad
Targeted Allele Detail

Summary

• Symbol: Myh10^tm3.1Rsad
• Name: myosin, heavy polypeptide 10, non-muscle; targeted mutation 3.1, Robert S Adelstein
• MGI ID: MGI:3052112
• Synonyms: 2BR709C, B^C, B^R709C
• Gene: Myh10 Location: Chr11:68582385-68707458 bp, + strand Genetic Position: Chr11, 41.95 cM, cytoband B3
• Alliance: Myh10^tm3.1Rsad page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:92081
• Parent Cell Line: CMT-1 (ES Cell)
• Strain of Origin: 129S6/SvEvTac

Mutation description

• Allele Type: Targeted (Humanized sequence)
• Mutation: Nucleotide substitutions
• Mutation details: Crossing Myh10^tm3Rsad mice with mice expressing cre in the germ line created this allele in which the floxed neo is excised, leaving a single loxP site upstream of exon 17 and the R709C mutation in exon 17. Western blot indicates the allele expression in mutant brains is restored to wild-type levels. (J:92081)

Expression

In Structures Affected by this Mutation:

11 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Myh10 Mutation: 95 strains or lines available

References

• Original: J:92081 Ma X, et al., A point mutation in the motor domain of nonmuscle myosin II-B impairs migration of distinct groups of neurons. Mol Biol Cell. 2004 Jun;15(6):2568-79
• All: 3 reference(s)