Lgr4^{Gt(pGT0TMpfs)1Wcs}
Gene trapped Allele Detail

Summary

• Symbol: Lgr4^{Gt(pGT0TMpfs)1Wcs}
• Name: leucine-rich repeat-containing G protein-coupled receptor 4; gene trap 1, William C Skarnes
• MGI ID: MGI:3052121
• Synonyms: Gpr48^Gt(pGT0)1Ah, LGR4^-
• Gene: Lgr4 Location: Chr2:109747992-109844602 bp, + strand Genetic Position: Chr2, 56.65 cM
• Alliance: Lgr4^{Gt(pGT0TMpfs)1Wcs} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:92077
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Gene trapped (Null/knockout, Reporter)
• Mutation: Insertion of gene trap vector
• Mutation details: A vector was inserted at the 5' end of intron 1. The vector contained the mouse En2 splice acceptor, CD4 transmembrane domain, Beta-geo, IRES, PLAP and SV40 polyA. PCR indicated loss of endogenous gene expression, but gain of a transcript of the endogenous LRRNT domain fused to Beta-geo. PLAP was also expressed. (J:92077)

Phenotypes

Key:

hm	homozygous	ht	heterozygous	tg	involves transgenes	√	phenotype observed
cn	conditional genotype	cx	complex: > 1 genome feature	ot	other: hemizygous, indeterminate,...	N	normal phenotype

Genotype/
Background:

	Allelic Composition	Genetic Background	Cell Line(s)
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hm1	Lgr4^Gt(pGT0TMpfs)1Wcs/Lgr4^Gt(pGT0TMpfs)1Wcs	involves: 129P2/Ola * C57BL/6
hm2	Lgr4^Gt(pGT0TMpfs)1Wcs/Lgr4^Gt(pGT0TMpfs)1Wcs	involves: 129P2/OlaHsd * C57BL/6 * CD-1
ht3	Lgr4^Gt(pGT0TMpfs)1Wcs/Lgr4^+	involves: 129P2/Ola * C57BL/6

Phenotypes:

Affected Systems	hm1		hm2		ht3
show or hide all annotated terms Sex:
adipose tissue			√	√
abnormal epididymal fat pad morphology			√	√
endocrine/exocrine glands		√		√
abnormal testis morphology				√
abnormal rete testis morphology				√
rete testis obstruction				√
abnormal seminiferous tubule epithelium morphology				√
enlarged testis		√
abnormal testis physiology				√
growth/size/body	√	√	√	√
epididymal cyst				√
decreased body size			√	√
decreased body weight	√	√	√	√
liver/biliary system	√	√
decreased liver weight	√	√
mortality/aging	√	√	√	√	√
perinatal lethality, incomplete penetrance	√	√			√
postnatal lethality, incomplete penetrance			√	√
renal/urinary system	√	√
abnormal kidney morphology	√	√
decreased kidney weight	√	√
reproductive system	√	√		√
abnormal testis morphology				√
abnormal rete testis morphology				√
rete testis obstruction				√
abnormal seminiferous tubule epithelium morphology				√
enlarged testis		√
abnormal testis physiology				√
enlarged uterus	√
abnormal efferent ductules of testis morphology				√
abnormal epididymis morphology				√
epididymal cyst				√
small caput epididymis				√
abnormal epididymis epithelium morphology				√
epididymis hypoplasia				√
infertility	√	√
male infertility				√
decreased epididymal cell proliferation				√

View phenotypes and curated references for all genotypes (concatenated display).

Expression

In Mice Carrying this Mutation:	4 assay results
In Structures Affected by this Mutation:	11 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Lgr4 Mutation: 94 strains or lines available

References

• Original: J:92077 Mazerbourg S, et al., Leucine-rich repeat-containing, g protein-coupled receptor 4 null mice exhibit intrauterine growth retardation associated with embryonic and perinatal lethality. Mol Endocrinol. 2004 Sep;18(9):2241-54
• All: 8 reference(s)