Ezr^tm1Aim
Targeted Allele Detail

Summary

• Symbol: Ezr^tm1Aim
• Name: ezrin; targeted mutation 1, Andrea I McClatchey
• MGI ID: MGI:3052158
• Synonyms: Ez^-, Ez^{delta345-Betageo}
• Gene: Ezr Location: Chr17:7005530-7050179 bp, - strand Genetic Position: Chr17, 4.38 cM
• Alliance: Ezr^tm1Aim page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:92210
• Parent Cell Line: J1 (ES Cell)
• Strain of Origin: 129S4/SvJae

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: LoxP sites were inserted to flank exons 3-5. An FRT-flanked promoterless Beta-galactosidase-neomycin cassette was inserted into intron 5. Crossing to EIIa-Cre mice excised the floxed region containing exons 3-5 and left the FRT-flanked Beta-geo. Immunohistochemical analysis of mutants confirmed absence of endogenous protein. Western blot of mutant neonate intenstinal tissue also demonstrated absence of protein. (J:92210)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Ezr Mutation: 87 strains or lines available

References

• Original: J:92210 Saotome I, et al., Ezrin is essential for epithelial organization and villus morphogenesis in the developing intestine. Dev Cell. 2004 Jun;6(6):855-64
• All: 2 reference(s)