Epor^{tm1.1(EGFP/icre)Uk}
Targeted Allele Detail

Summary

• Symbol: Epor^{tm1.1(EGFP/icre)Uk}
• Name: erythropoietin receptor; targeted mutation 1.1, Ursula Klingmuller
• MGI ID: MGI:3052727
• Synonyms: EpoRCre, EpoR-iCre, Epor^{tm1.1(EGFP/icre)Uk}, ErGFPcre
• Gene: Epor Location: Chr9:21870193-21874802 bp, - strand Genetic Position: Chr9, 7.93 cM
• Alliance: Epor^{tm1.1(EGFP/icre)Uk} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:92235
• Parent Cell Line: BALB/c-I (ES Cell)
• Strain of Origin: BALB/cJ

Mutation description

• Allele Type: Targeted (Null/knockout, Recombinase, Reporter)
• Mutation: Insertion
• Epor^{tm1.1(EGFP/icre)Uk} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Epor (MGI:95408)

• Mutation details: An EGFP/icre sequence was inserted into exon 1, replacing the endogenous ATG with the translational start site of the EGFP/icre, yet preserving the last 10 bp of the splice-donor site of exon 1. A FRT flanked neo was inserted downstream to replace parts of exon 2 and exons 3-8. Transient FLP expression excised the neo. Southern blot and multiplex PCR confirmed recombination in heterozygous mice. Immunoblot of FLCs demonstrated green fluorescence in heterozygous and homozygous embryos, with homozygotes showing a 2-fold increase in fluorescence intensity compared to heterozygotes. (J:92235)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Epor (mouse) Summary of all recombinase alleles driven by Epor. Comparative matrix view of recombinase activities Alliance mouse Epor gene page

Expression

In Mice Carrying this Mutation:

7 assay results 4 RNA-Seq or microarray experiment(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Epor Mutation: 26 strains or lines available

References

• Original: J:92235 Heinrich AC, et al., A mouse model for visualization and conditional mutations in the erythroid lineage. Blood. 2004 Aug 1;104(3):659-66
• All: 76 reference(s)