Tert^tm1Yjc
Targeted Allele Detail

Summary

• Symbol: Tert^tm1Yjc
• Name: telomerase reverse transcriptase; targeted mutation 1, Y Jeffrey Chiang
• MGI ID: MGI:3052737
• Synonyms: mTERT^-, TERT^-
• Gene: Tert Location: Chr13:73775030-73797962 bp, + strand Genetic Position: Chr13, 40.12 cM
• Alliance: Tert^tm1Yjc page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:92243
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Insertion
• Mutation details: A GFPneo was inserted into the 5' UTR of exon 1. Southern blot and PCR confirmed recombination in mutant mice. RT-PCR showed a lack of transcript in mutants and a telomerase repeat amplification (TRAP) assay indicated there was no telomerase activity in testis cell extracts from mutants. (J:92243)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Tert Mutation: 55 strains or lines available

References

• Original: J:92243 Chiang YJ, et al., Expression of telomerase RNA template, but not telomerase reverse transcriptase, is limiting for telomere length maintenance in vivo. Mol Cell Biol. 2004 Aug;24(16):7024-31
• All: 19 reference(s)