Parg^tm1Zqw
Targeted Allele Detail

Summary

• Symbol: Parg^tm1Zqw
• Name: poly (ADP-ribose) glycohydrolase; targeted mutation 1, Zhao-Qi Wang
• MGI ID: MGI:3052740
• Synonyms: Parg(110), Parg(110)^-, Parg^delta2-3
• Gene: Parg Location: Chr14:31923906-32019507 bp, + strand Genetic Position: Chr14, 19.4 cM, cytoband B
• Alliance: Parg^tm1Zqw page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:92244
• Parent Cell Line: E14.1 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Modified isoform(s))
• Mutations: Insertion, Intragenic deletion
• Mutation details: Exons 2 and 3 were excised following transient Cre expression, leaving a single loxP site in intron 1. Western blot showed only a 60 kDa protein was present in mutants and wild-type, while wild-type also had a 110 kDa protein. RT-PCR confirmed that exons 2 and 3 had been deleted. RACE-PCR indicated presence of 2 variant transcripts in mutants: one transcript including exon 1 and exons 4 to 18 and the other only exons 4-18. (J:92244)

Expression

In Mice Carrying this Mutation:	2 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Parg Mutation: 68 strains or lines available

References

• Original: J:92244 Cortes U, et al., Depletion of the 110-kilodalton isoform of poly(ADP-ribose) glycohydrolase increases sensitivity to genotoxic and endotoxic stress in mice. Mol Cell Biol. 2004 Aug;24(16):7163-78
• All: 8 reference(s)