Foxo1^tm1Tf
Targeted Allele Detail

Summary

• Symbol: Foxo1^tm1Tf
• Name: forkhead box O1; targeted mutation 1, Tatsuo Furuyama
• MGI ID: MGI:3052743
• Synonyms: Foxo1^-
• Gene: Foxo1 Location: Chr3:52175758-52257530 bp, + strand Genetic Position: Chr3, 23.19 cM
• Alliance: Foxo1^tm1Tf page

Growth retardation, abnormal development of branchial arches and pericardial swelling in Foxo1^tm1Tf/Foxo1^tm1Tf mice

Show the 2 phenotype image(s) involving this allele.

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:92247
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Constitutively active)
• Mutations: Insertion, Intragenic deletion
• Mutation details: The 5' UTR and the first 208 codons of exon 1, encoding the N-terminal half of the forkhead domain, were replaced with a targeting vector containing a floxed PGK-neo and FOXO1a cDNA, encoding a constitutively active form of the protein. RT-PCR and Western blot showed lack of wild-type transcript and protein, respectively, in mutant embryos. (J:92247)

Expression

In Structures Affected by this Mutation:

6 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Foxo1 Mutation: 32 strains or lines available

References

• Original: J:92247 Furuyama T, et al., Abnormal angiogenesis in Foxo1 (Fkhr)-deficient mice. J Biol Chem. 2004 Aug 13;279(33):34741-9
• All: 6 reference(s)