Ubr5^tm1Ckww
Targeted Allele Detail

Summary

• Symbol: Ubr5^tm1Ckww
• Name: ubiquitin protein ligase E3 component n-recognin 5; targeted mutation 1, Colin K W Watts
• MGI ID: MGI:3052764
• Synonyms: Edd^delta
• Gene: Ubr5 Location: Chr15:37967572-38079098 bp, - strand Genetic Position: Chr15, 15.1 cM
• Alliance: Ubr5^tm1Ckww page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:92269
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129X1/SvJ

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A targeting vector was created to replace 60 bp of exon 1, encoding amino acids 2-21, and approximately 3.3 kb of intron 1 with a Betagal-GFP and PGK-neo. PCR confirmed recombination in mutants. Western blot indicated lack of protein in mutant embryos. (J:92269)

Expression

In Structures Affected by this Mutation:

4 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Ubr5 Mutation: 117 strains or lines available

References

• Original: J:92269 Saunders DN, et al., Edd, the murine hyperplastic disc gene, is essential for yolk sac vascularization and chorioallantoic fusion. Mol Cell Biol. 2004 Aug;24(16):7225-34
• All: 1 reference(s)