Prnp^tm1Tuzi
Targeted Allele Detail

Summary

• Symbol: Prnp^tm1Tuzi
• Name: prion protein; targeted mutation 1, Nadia L Tuzi
• MGI ID: MGI:3052798
• Synonyms: Prp^stop
• Gene: Prnp Location: Chr2:131751848-131780349 bp, + strand Genetic Position: Chr2, 64.07 cM
• Alliance: Prnp^tm1Tuzi page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:92302
• Parent Cell Line: HM-1 (ES Cell)
• Strain of Origin: 129P2/OlaHsd-Hprt1^b-m3

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Insertion
• Mutation details: A floxed Betageo cassette was inserted into intron 2. PCR confirmed recombination in mice. Northern blot demonstrated that transcription was abolished and Western blot indicated a complete absence of protein in mutant brains. (J:92302)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Prnp Mutation: 142 strains or lines available

References

• Original: J:92302 Tuzi NL, et al., Cre-loxP mediated control of PrP to study transmissible spongiform encephalopathy diseases. Genesis. 2004 Sep;40(1):1-6
• All: 2 reference(s)