Alb^{tm1(cre/ERT2)Mtz}
Targeted Allele Detail

Summary

• Symbol: Alb^{tm1(cre/ERT2)Mtz}
• Name: albumin; targeted mutation 1, Daniel Metzger
• MGI ID: MGI:3052812
• Synonyms: Alb-CreERT2, AlbCre-ERT2^ki, Alb^ICE, Alb^{tm1(cre/ESR1)Mtz}, Albumin-CreER, SA^Cre-ERT2, SA-CRE-ER^T2
• Gene: Alb Location: Chr5:90608756-90624461 bp, + strand Genetic Position: Chr5, 44.7 cM
• Alliance: Alb^{tm1(cre/ERT2)Mtz} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:92298
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129S2/SvPas

Mutation description

• Allele Type: Targeted (Inducible, Recombinase)
• Inducer: tamoxifen
• Mutation: Insertion
• Alb^{tm1(cre/ERT2)Mtz} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Alb (MGI:87991)

• Mutation details: A vector was used to insert IRES-Cre-ER^T2 downstream of the stop codon in exon 14. Transient FLP-recombinase excised a FRT-floxed neo 3' to the Cre. ES cell clone XF136.64 was injected into blastocysts to generated mice. Southern blot confirmed recombination. RT-PCR indicated bicistronic expression was only present in the liver of mutants. Immunohistochemical analysis of liver using an anti-Cre antibody showed that Cre is expressed in most, if not all, hepatocytes. (J:92298)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Alb (mouse) Summary of all recombinase alleles driven by Alb. Comparative matrix view of recombinase activities Alliance mouse Alb gene page

Disease models

Tumor Data

List all tumor models in MMHCdb carrying Alb^{tm1(cre/ERT2)Mtz}

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Alb Mutation: 97 strains or lines available

References

• Original: J:92298 Schuler M, et al., Efficient temporally controlled targeted somatic mutagenesis in hepatocytes of the mouse. Genesis. 2004 Jul;39(3):167-72
• All: 104 reference(s)