Trbv14^tm2Che
Targeted Allele Detail

Summary

• Symbol: Trbv14^tm2Che
• Name: T cell receptor beta, variable 14; targeted mutation 2, Jianzhu Chen
• MGI ID: MGI:3052833
• Synonyms: P13^R
• Gene: Trbv14 Location: Chr6:41112101-41112550 bp, + strand Genetic Position: Chr6, 19.22 cM
• Alliance: Trbv14^tm2Che page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:92288
• Parent Cell Line: J1 (ES Cell)
• Strain of Origin: 129S4/SvJae

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A targeting vector was constructed to replace promoter and the transcription start site, but not the translation start codon, with the SV40 minimal promoter plus five copies of the Gal4 DNA sequences. Flow cytometry showed mutant mice had very few T cells expressing the antibody. (J:92288)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Trbv14 Mutation: 7 strains or lines available

References

• Original: J:92288 Ryu CJ, et al., The T-cell receptor beta variable gene promoter is required for efficient V beta rearrangement but not allelic exclusion. Mol Cell Biol. 2004 Aug;24(16):7015-23
• All: 2 reference(s)