Tg(BCL2)25Wehi
Transgene Detail

Summary

• Symbol: Tg(BCL2)25Wehi
• Name: transgene insertion 25, Walter and Eliza Hall Institute of Medical Research
• MGI ID: MGI:3052945
• Synonyms: 25Wehi, Bcl2/Wehi25 Tg, Emu-2-25, Emu-bcl-2-25, H2^k-Bcl2-tg, hBcl-2
• Transgene: Tg(BCL2)25Wehi Location: unknown
• Alliance: Tg(BCL2)25Wehi page

Transgene origin

• Strain of Origin: (C57BL/6JWehi x SJL/JWehi)F2

Transgene description

• Transgene Type: Transgenic (Humanized sequence, Inserted expressed sequence)
• Mutation: Insertion
• Tg(BCL2)25Wehi expresses 1 gene
  Transgene expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  BCL2 (596)

  Bcl2	(MGI:88138)

• Mutation details: The transgene construct consists of the human BCL2 cDNA in association with the Emu immunoglobulin heavy chain enhancer and SV40 promoter. (J:93111)

Tumor Data

List all tumor models in MMHCdb carrying Tg(BCL2)25Wehi

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

Notes

In transgenic mice on a C57BL/6 background, expression of the transgene is restricted to the T cell lineage (no B-cell expression).

Thymocytes, peripheral T-cells and activated T cells from these mice withstand prolonged culture in the absence of growth factors. Cells are resistant to killing by gamma-radiation, glucocorticoids, ionomycin, PMA, and sodium azide, but not complement, cytotoxic T cells or Fas ligand. Hemizygotes have a normal total T-cell count and thymic involution rate but show an enhanced response to immunization. This transgenic line displays no detectable autoimmunity. These mice serve as a robust source for the production of T-cell lines or hybridomas.

References

• Original: J:93111 Strasser A, et al., Abnormalities of the immune system induced by dysregulated bcl-2 expression in transgenic mice. Curr Top Microbiol Immunol. 1990;166:175-81
• All: 59 reference(s)