Tg(TcrAND)53Hed
Transgene Detail

Summary

• Symbol: Tg(TcrAND)53Hed
• Name: transgene insertion 53, Stephen M Hedrick
• MGI ID: MGI:3053044
• Synonyms: AND, AND TCR, CC, TcrAND, Tg(TcrAND)
• Transgene: Tg(TcrAND)53Hed Location: unknown
• Alliance: Tg(TcrAND)53Hed page

Transgene origin

• Strain of Origin: (C57BL/6 x SJL)F2

Transgene description

• Transgene Type: Transgenic (Inserted expressed sequence)
• Mutation: Insertion
• Tg(TcrAND)53Hed expresses 2 genes
  Transgene expresses:
  

  Organism	Expressed Gene	Note
  mouse	Tcra (MGI:98553)
  mouse	Tcrb (MGI:98578)

• Mutation details: Rearranged TCR alpha and beta genes containing endogenous promoter and enhancer sequences were coinjected into the micronucleus of 1 cell embryos. Both constructs are specific for the carboxyterminal fragment of pigeon cytochrome c and the E^k molecule. The alpha-chain of this transgenic TCR differs by one amino acid (an alanine instead of a threonine at position 99) to the alpha chain of the transgenic TCR expressed in Tg(TcraTcrbAD10)1Hed transgenic mice. (J:85777)

Disease models

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available

Notes

The transgenic TCR is expressed on virtually all T cells in mice expressing E^k. Transgenic mice on a background that involves C57BL/10, C57BL/6, and SJL show expression of a rearranged T-cell receptor specific for the carboxy-terminal fragment of pigeon cytochrome c and the E^k molecule, resulting in a major subpopulation of T cells restricted to class II MHC proteins. These mice have an abnormally high percentage of mature CD4⁺CD8^- cells, a peripheral T-cell population is almost exclusively CD4⁺ and show no expression of I-E^k.

References

• Original: J:85777 Kaye J, et al., Selective development of CD4+ T cells in transgenic mice expressing a class II MHC-restricted antigen receptor. Nature. 1989 Oct 26;341(6244):746-9
• All: 185 reference(s)