Noto^tm1Gos
Targeted Allele Detail

Summary

• Symbol: Noto^tm1Gos
• Name: notochord homeobox; targeted mutation 1, Achim Gossler
• MGI ID: MGI:3053092
• Synonyms: Not^eGFP, Noto^GFP
• Gene: Noto Location: Chr6:85400868-85405859 bp, + strand Genetic Position: Chr6, 37.44 cM
• Alliance: Noto^tm1Gos page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:91405
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129S1/SvImJ

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: An eGFP and floxed neo were inserted in frame into exon 1, deleting most of exon 1 and all of exon 2, thereby removing about 80% of the open reading frame, including the homeodomain. The floxed neo was excised by crossing mice with mice expressing cre in the germ line. PCR confirmed recombination in mutant mice. (J:91405)

Expression

In Mice Carrying this Mutation:	206 assay results 1 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	3 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Noto Mutation: 15 strains or lines available

References

• Original: J:91405 Abdelkhalek HB, et al., The mouse homeobox gene Not is required for caudal notochord development and affected by the truncate mutation. Genes Dev. 2004 Jul 15;18(14):1725-36
• All: 10 reference(s)