Ly96^tm1Kmiy
Targeted Allele Detail

Summary

• Symbol: Ly96^tm1Kmiy
• Name: lymphocyte antigen 96; targeted mutation 1, Kensuke Miyake
• MGI ID: MGI:3053109
• Synonyms: Ly96-, Md2^-, MD-2^-
• Gene: Ly96 Location: Chr1:16758680-16779829 bp, + strand Genetic Position: Chr1, 5.1 cM
• Alliance: Ly96^tm1Kmiy page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:92494
• Parent Cell Line: E14.1 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A targeting vector was designed to replace the first exon, encoding 37 amino acids, with a neomycin resistance gene. RT-PCR indicated homozygotes lacked mRNA in bone marrow-derived macrophages. Protein was not expressed on the cell surface as shown by staining with a mAB for the TLR4-Ly96 complex. (J:92494)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Ly96 Mutation: 13 strains or lines available

References

• Original: J:92494 Nagai Y, et al., Essential role of MD-2 in LPS responsiveness and TLR4 distribution. Nat Immunol. 2002 Jul;3(7):667-72
• All: 51 reference(s)