Kcnma1^tm1Rwa
Targeted Allele Detail

Summary

• Symbol: Kcnma1^tm1Rwa
• Name: potassium large conductance calcium-activated channel, subfamily M, alpha member 1; targeted mutation 1, Richard W Aldrich
• MGI ID: MGI:3053849
• Synonyms: KCa1.1^-, Slo^-
• Gene: Kcnma1 Location: Chr14:23342356-24055173 bp, - strand Genetic Position: Chr14, 12.92 cM
• Alliance: Kcnma1^tm1Rwa page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:92539
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: LoxP sites were inserted to flank exon 1. In addition, EGFP-FRT-flanked neomycin cassette was inserted 3' to exon 1. The floxed exon was removed by mating mutant animals to the cre deleter strain FVB/N-Tg(EIIa-cre)C5379Lmgd/J. Protein was undetectable by Western blot analysis of bladder proteins. The EGFP-neomycin cassette was not removed by Cre recombinase. (J:92539)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Kcnma1 Mutation: 101 strains or lines available

References

• Original: J:92539 Meredith AL, et al., Overactive bladder and incontinence in the absence of the BK large conductance Ca2+-activated K+ channel. J Biol Chem. 2004 Aug 27;279(35):36746-52
• All: 32 reference(s)