Mal^tm1Nsw
Targeted Allele Detail

Summary

• Symbol: Mal^tm1Nsw
• Name: myelin and lymphocyte protein, T cell differentiation protein; targeted mutation 1, Nicole Shaeren-Wiemers
• MGI ID: MGI:3053860
• Synonyms: Mal^-
• Gene: Mal Location: Chr2:127475146-127498615 bp, - strand Genetic Position: Chr2, 62.06 cM
• Alliance: Mal^tm1Nsw page

Compact myelin of Mal^tm1Nsw/Mal^tm1Nsw mice contains cytoplasmic inclusions

Show the 2 phenotype image(s) involving this allele.

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:92517
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A lacZ/neo cassette was inserted in frame after the third codon in exon 1, replacing the remainder of exon 1. Southern blot confirmed recombination in mutant mice. Northern blot indicated a lack of full-length transcript in homozygotes, but presence of a lacZ-containing transcript in homozygotes and heterozygotes. Absence of wild-type protein was demonstrated by an immunohistochemistry assay of sciatic nerves and brain from mutants. (J:92517)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Mal Mutation: 19 strains or lines available

References

• Original: J:92517 Schaeren-Wiemers N, et al., The raft-associated protein MAL is required for maintenance of proper axon--glia interactions in the central nervous system. J Cell Biol. 2004 Aug 30;166(5):731-42
• All: 6 reference(s)