Col19a1^tm1Rmz
Targeted Allele Detail

Summary

• Symbol: Col19a1^tm1Rmz
• Name: collagen, type XIX, alpha 1; targeted mutation 1, Francesco Ramirez
• MGI ID: MGI:3053870
• Synonyms: N19
• Gene: Col19a1 Location: Chr1:24300971-24626553 bp, - strand Genetic Position: Chr1, 9.99 cM, cytoband A3
• Alliance: Col19a1^tm1Rmz page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:92510
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A PGK-neo cassette replaced exon 4, which codes for 32 internal amino acids of the 268-residue NC6 peptide and includes split codons for the first and last residues. Northern blot demonstrated lack of mRNA transcripts in homozygotes. Sequencing of RT-PCR products showed that there were no shorter in-frame transcripts present. Immunoblots demonstrated the absence of the expected 165-kD collagenase-sensitive product. (J:92510)

Expression

In Structures Affected by this Mutation:

3 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Col19a1 Mutation: 81 strains or lines available

References

• Original: J:92510 Sumiyoshi H, et al., Esophageal muscle physiology and morphogenesis require assembly of a collagen XIX-rich basement membrane zone. J Cell Biol. 2004 Aug 16;166(4):591-600
• All: 2 reference(s)