Col19a1^tm2Rmz
Targeted Allele Detail

Summary

• Symbol: Col19a1^tm2Rmz
• Name: collagen, type XIX, alpha 1; targeted mutation 2, Francesco Ramirez
• MGI ID: MGI:3053871
• Synonyms: Delta19
• Gene: Col19a1 Location: Chr1:24300971-24626553 bp, - strand Genetic Position: Chr1, 9.99 cM, cytoband A3
• Alliance: Col19a1^tm2Rmz page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:92510
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A PGK-neo replaced exons 38-40, which encode the 20-residue NC3 interruption of the helical domain and one and six collagenous tripeptides located amino-and carboxyl-terminally, respectively. Sequencing of RT-PCR products confirm that the mutant transcript is in frame. Immunoblots identified a collagen-sensitive protein in mutant tissue slightly smaller than that of wild-type protein. PCR amplification indicated that mutant and wild-type transcripts are present at comparable levels in heterozygotes. (J:92510)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Col19a1 Mutation: 81 strains or lines available

References

• Original: J:92510 Sumiyoshi H, et al., Esophageal muscle physiology and morphogenesis require assembly of a collagen XIX-rich basement membrane zone. J Cell Biol. 2004 Aug 16;166(4):591-600
• All: 1 reference(s)