Nr5a2^tm1Auw
Targeted Allele Detail

Summary

• Symbol: Nr5a2^tm1Auw
• Name: nuclear receptor subfamily 5, group A, member 2; targeted mutation 1, Johan Auwerx
• MGI ID: MGI:3053912
• Synonyms: LRH-1^-
• Gene: Nr5a2 Location: Chr1:136770309-136888186 bp, - strand Genetic Position: Chr1, 59.98 cM, cytoband E4
• Alliance: Nr5a2^tm1Auw page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:92508
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Amino acids 283-295, coded by exon 6, were replaced with an IRES-lacZ and neo cassette. Correct targeting was confirmed by Southern blot analysis. Quantitative PCR of heterozygotes showed a mean reduction of 40% in mRNA levels in the duodenum, ileum, and colon relative to wild-type mice. (J:92508)

Expression

In Structures Affected by this Mutation:

6 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Nr5a2 Mutation: 96 strains or lines available

References

• Original: J:92508 Botrugno OA, et al., Synergy between LRH-1 and beta-catenin induces G1 cyclin-mediated cell proliferation. Mol Cell. 2004 Aug 27;15(4):499-509
• All: 9 reference(s)