Gli1^{tm3(cre/ERT2)Alj}
Targeted Allele Detail

Summary

• Symbol: Gli1^{tm3(cre/ERT2)Alj}
• Name: GLI-Kruppel family member GLI1; targeted mutation 3, Alexandra L Joyner
• MGI ID: MGI:3053957
• Synonyms: Gli1^CreER, Gli1-CreER^T2, Gli1^CreERT2, Gli1^{tm3(cre/ERT2)Alj}, Gli1^{tm3(cre/ESR1)Alj}
• Gene: Gli1 Location: Chr10:127165751-127177448 bp, - strand Genetic Position: Chr10, 74.5 cM
• Alliance: Gli1^{tm3(cre/ERT2)Alj} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:92507
• Parent Cell Line: W4 (ES Cell)
• Strain of Origin: 129S6/SvEvTac

Mutation description

• Allele Type: Targeted (Inducible, Recombinase)
• Inducer: tamoxifen
• Mutation: Insertion
• Gli1^{tm3(cre/ERT2)Alj} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Gli1 (MGI:95727)

• Mutation details: CreERT2 cDNA and a floxed neo were inserted into the 5' UTR of the first coding exon. Crossing with mice expressing Cre removed the floxed neo. Cre expression in mice carrying this allele is induced by treating the mice with tamoxifen. (J:92507)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Gli1 (mouse) Summary of all recombinase alleles driven by Gli1. Comparative matrix view of recombinase activities Alliance mouse Gli1 gene page

Phenotypes

Key:

hm	homozygous	ht	heterozygous	tg	involves transgenes	√	phenotype observed
cn	conditional genotype	cx	complex: > 1 genome feature	ot	other: hemizygous, indeterminate,...	N	normal phenotype

Genotype/
Background:

	Allelic Composition	Genetic Background	Cell Line(s)

	Allelic Composition	Genetic Background	Cell Line(s)
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cn1	Ctnnb1^tm4Wbm/Ctnnb1^tm4Wbm Gli1^tm3(cre/ERT2)Alj/Gli1^+	involves: 129P2/OlaHsd * 129S6/SvEvTac
cn2	Gli1^tm3(cre/ERT2)Alj/Gli1^+ Gt(ROSA)26Sor^tm4(ACTB-tdTomato,-EGFP)Luo/Gt(ROSA)26Sor^+ Tnn^tm1b(KOMP)Wtsi/Tnn^tm1b(KOMP)Wtsi	involves: 129S1/Sv * 129S6/SvEvTac * 129X1/SvJ * C57BL/6N
cn3	Gt(ROSA)26Sor^tm2(HOXC9)Odk/Gt(ROSA)26Sor^tm2(HOXC9)Odk Gli1^tm3(cre/ERT2)Alj/Gli1^+	involves: 129S6/SvEvTac
cn4	Gli1^tm3(cre/ERT2)Alj/Gli1^+ Gt(ROSA)26Sor^tm1(Smo/EYFP)Amc/Gt(ROSA)26Sor^+	involves: 129S6/SvEvTac * 129X1/SvJ
cn5	Gli1^tm3(cre/ERT2)Alj/Gli1^+ Gt(ROSA)26Sor^tm1(Smo/EYFP)Amc/Gt(ROSA)26Sor^tm1(Smo/EYFP)Amc	involves: 129S6/SvEvTac * 129X1/SvJ
cn6	Ctnnb1^tm1Mmt/Ctnnb1^tm1Mmt Gli1^tm3(cre/ERT2)Alj/Gli1^+	involves: 129S6/SvEvTac * 129X1/SvJ
cn7	Slfn4^tm1c(EUCOMM)Wtsi/Slfn4^tm1c(EUCOMM)Wtsi Gli1^tm3(cre/ERT2)Alj/Gli1^+	involves: 129S6/SvEvTac * Black Swiss * C57BL/6 * Swiss Webster	EPD0746_4_G08
cn8	Ar^tm2Ska/Y Gli1^tm3(cre/ERT2)Alj/Gli1^+	involves: 129S6/SvEvTac * C57BL/6 * CBA

Phenotypes:

Affected Systems	cn1	cn2	cn3	cn4	cn5	cn6	cn7	cn8
show or hide all annotated terms Sex:
craniofacial		√	N
craniofacial phenotype			N
abnormal tooth development		√
digestive/alimentary system							√
abnormal stomach mucosa morphology							√
growth/size/body		√
abnormal tooth development		√
hematopoietic system							√
abnormal CD8-positive, alpha-beta cytotoxic T cell morphology							√
homeostasis/metabolism							√
abnormal interleukin level							√
abnormal tumor necrosis factor level							√
immune system							√
abnormal CD8-positive, alpha-beta cytotoxic T cell morphology							√
abnormal interleukin level							√
abnormal tumor necrosis factor level							√
decreased susceptibility to bacterial infection							√
mortality/aging					√
premature death					√
neoplasm					√
increased medulloblastoma incidence					√
nervous system					√
increased medulloblastoma incidence					√
renal/urinary system	√							√
abnormal foreskin morphology	√							√
reproductive system	√					√		√
abnormal external female genitalia morphology						√
abnormal external male genitalia morphology	√							√
abnormal foreskin morphology	√							√
abnormal reproductive system development								√
respiratory system				N
respiratory system phenotype				N
skeleton		√
abnormal tooth development		√

View phenotypes and curated references for all genotypes (concatenated display).

Tumor Data

List all tumor models in MMHCdb carrying Gli1^{tm3(cre/ERT2)Alj}

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Gli1 Mutation: 50 strains or lines available

References

• Original: J:92507 Ahn S, et al., Dynamic changes in the response of cells to positive hedgehog signaling during mouse limb patterning. Cell. 2004 Aug 20;118(4):505-16
• All: 231 reference(s)