Tg(MMTVtTA)1Mam
Transgene Detail

Summary

• Symbol: Tg(MMTVtTA)1Mam
• Name: transgene insertion 1, Lothar Hennighausen
• MGI ID: MGI:3053958
• Synonyms: MMTV-tTA
• Transgene: Tg(MMTVtTA)1Mam Location: unknown
• Alliance: Tg(MMTVtTA)1Mam page

Transgene origin

• Strain of Origin: C57BL/6 and SJL

Transgene description

• Transgene Type: Transgenic (Transactivator)
• Mutation: Insertion
• Mutation details: The MMTV LTR was used to target expression of tetracycline-controlled transactivator protein (tTA) to the epithelial cells of secretory organs and skin. The tetracycline resistance gene (TetR) was fused at the C-terminus with the viral co-activator, virion protein 16 of the herpes simplex virus (VP-16). (J:92586)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 3 strains available Cell Lines: 0 lines available

Notes

Additional lines were generated and showed comparable tissue expression patterns.

When transgenic mice were mated to mice transgenic for either lacZ or luciferase reporter genes coupled to tetracycline-responsive promoter elements (TRE), nearly uniform expression of the reporter was found in seminal vesicle, salivary gland, and Leydig cells of the double transgenic offspring. More heterogeneous patterns of reporter expression were observed in mammary epithelial cells and basal cells of the epidermis. Lower levels of reporter gene expression were also observed in a broad range of tissues. Transcriptional activation mediated by tTA was up to several hundredfold and was abrogated after the administration of tetracycline.

In combination with Tg(BCR/ABL1)2Dgt, when tetracycline administration is stopped, bitransgenic mice are models for B cell acute lymphoblastic leukemia (ALL). (J:72377)

References

• Original: J:92586 Hennighausen L, et al., Conditional gene expression in secretory tissues and skin of transgenic mice using the MMTV-LTR and the tetracycline responsive system. J Cell Biochem. 1995 Dec;59(4):463-72
• All: 23 reference(s)