Cdk6^tm1Bbd
Targeted Allele Detail

Summary

• Symbol: Cdk6^tm1Bbd
• Name: cyclin dependent kinase 6; targeted mutation 1, Mariano Barbacid
• MGI ID: MGI:3053975
• Synonyms: Cdk6^-
• Gene: Cdk6 Location: Chr5:3391485-3581008 bp, + strand Genetic Position: Chr5, 2.04 cM, cytoband A2-A3
• Alliance: Cdk6^tm1Bbd page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:92529
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A PGK-neo cassette replaced the first coding exon, which contains the ATG as well as the PLSTIRE domain essential for cyclin binding. PCR confirmed recombination in mutant mice. Immunohistochemical analysis of lymphocytes indicated lack of protein expression in mutants. (J:92529)

Disease models

Expression

In Mice Carrying this Mutation:	1 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	7 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Cdk6 Mutation: 40 strains or lines available

References

• Original: J:92529 Malumbres M, et al., Mammalian cells cycle without the D-type cyclin-dependent kinases Cdk4 and Cdk6. Cell. 2004 Aug 20;118(4):493-504
• All: 18 reference(s)