Tg(EPO33)72Ptc
Transgene Detail

Summary

• Symbol: Tg(EPO33)72Ptc
• Name: transgene insertion 72, Peter Curtin
• MGI ID: MGI:3054249
• Transgene: Tg(EPO33)72Ptc Location: unknown
• Alliance: Tg(EPO33)72Ptc page

Transgene origin

• Strain of Origin: C57BL/6J x SJL/J

Transgene description

• Transgene Type: Transgenic (Humanized sequence, Inserted expressed sequence)
• Mutation: Insertion
• Tg(EPO33)72Ptc expresses 1 gene
  Transgene expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  EPO (2056)

  Epo	(MGI:95407)

• Mutation details: The transgene contains a mitochondrial uncoupling protein promoter (Ucp) and a 33 kb genomic fragment including 4 kb of human erythropoietin (EPO) along with 20 kb of 5' flanking sequence and 8.5 kb of 3' flanking sequence. Several lines were created. Gene copy number in line 72 is 2. (J:92650)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

Notes

Anemia produced by phelebotomy or treatment with phenylhydrazine induced the transgene in kidney and liver with some variability; cobalt and hypoxia also induced the transgene. In the absence of induced anemia, constitutive expression was not found in brain, heart, kidney, spleen and liver.

References

• Original: J:92650 Madan A, et al., Regulated basal, inducible, and tissue-specific human erythropoietin gene expression in transgenic mice requires multiple cis DNA sequences. Blood. 1995 May 15;85(10):2735-41
• All: 1 reference(s)