Nt5e^tm1Jgsc
Targeted Allele Detail

Summary

• Symbol: Nt5e^tm1Jgsc
• Name: 5' nucleotidase, ecto; targeted mutation 1, Jurgen Schnermann
• MGI ID: MGI:3054787
• Synonyms: e-5'NT/CD73^-
• Gene: Nt5e Location: Chr9:88209662-88254142 bp, + strand Genetic Position: Chr9, 47.24 cM, cytoband E3.2
• Alliance: Nt5e^tm1Jgsc page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:92598
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129S/SvEv

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Exons 2 and 3, encoding the presumptive catalyitic site, were replaced with a neomycin resistance gene. RT-PCR demonstrated a lack of transcript in mutants. Immunoperoxidase labeling against the endogenous protein confirmed its absence in kidneys. (J:92598)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Nt5e Mutation: 30 strains or lines available

References

• Original: J:92598 Castrop H, et al., Impairment of tubuloglomerular feedback regulation of GFR in ecto-5'-nucleotidase/CD73-deficient mice. J Clin Invest. 2004 Sep;114(5):634-42
• All: 12 reference(s)