Cd22^tm3Tft
Targeted Allele Detail

Summary

• Symbol: Cd22^tm3Tft
• Name: CD22 antigen; targeted mutation 3, Thomas F Tedder
• MGI ID: MGI:3054888
• Synonyms: Cd22delta1-2
• Gene: Cd22 Location: Chr7:30564829-30579767 bp, - strand Genetic Position: Chr7, 19.26 cM
• Alliance: Cd22^tm3Tft page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:92736
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: Exons 3 and 4 were deleted following Cre-mediated excision of a floxed neo. A 3' mRNA splice junction of exon 3 was retained. Immunoblot and flow cytometry confirmed protein expression by B cells. Protein migrated as a 120 kDa fragment, the predicted size of the protein lacking two immunoglobulin-like domains. (J:92736)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Cd22 Mutation: 64 strains or lines available

References

• Original: J:92736 Poe JC, et al., CD22 regulates B lymphocyte function in vivo through both ligand-dependent and ligand-independent mechanisms. Nat Immunol. 2004 Oct;5(10):1078-87
• All: 3 reference(s)