Tg(TcraTcrb)1100Mjb
Transgene Detail

Summary

• Symbol: Tg(TcraTcrb)1100Mjb
• Name: transgene insertion 1100, Michael J Bevan
• MGI ID: MGI:3054907
• Synonyms: OT-1, OT1, OT1 TCR, OT-I, OTI, OT-I(OVA)TCR, OT-I TCR, OT-ITCR, OVA-tcr-I, OVA-Tg, TCR-OT-I, Tg(OT-I), Tg(TcrOva)1100Mjb, Tg(TcrOVA)1100 mjb
• Transgene: Tg(TcraTcrb)1100Mjb Location: unknown Genetic Position: Chr3, Syntenic
• Alliance: Tg(TcraTcrb)1100Mjb page

Transgene origin

• Strain of Origin: C57BL/6

Transgene description

• Transgene Type: Transgenic (Inserted expressed sequence)
• Mutation: Insertion
• Tg(TcraTcrb)1100Mjb expresses 2 genes
  Transgene expresses:
  

  Organism	Expressed Gene	Note
  mouse	Tcra (MGI:98553)
  mouse	Tcrb (MGI:98578)

• Mutation details: Transgenic mice carry two coinjected constructs containing rearranged mouse TCR alpha (Tcra-V2, Tcra-J(alpha26)) and TCR beta (Tcrb-V5, Tcrb-D(beta2), Tcrb-J(beta2.6)) chains isolated from T cell clone 149.42. This transgenic T cell receptor recognizes ovalbumin residues 257-264 (SIINFEKL) in the context of H2K^b. The rearranged Tcra (alpha chain) cDNA specific for ovalbumin was isolated from T cells (clone AD10) and is expressed under the control of a 2-kb fragment of the H-2Kb promoter, a 700-bp fragment including the Ig H chain enhancer located 5' to the promoter, and a 1.7-kb BamHI-PstI fragment containing the 3' half of the human adult 8-globin gene (including part of exon 2, the exon 2-exon 3 C.F6 TCR a-chain gene that was truncated at the 3' XbaI site intron, exon 3) and polyadenylation signal. The Tcrb (beta chain) construct consists of a genomic fragment containing the Tcrb coding and endogenous promoter regions from a T cell clone (B3) specific for ovalbumin. (J:92867)

Tumor Data

List all tumor models in MMHCdb carrying Tg(TcraTcrb)1100Mjb

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 6 strains available Cell Lines: 0 lines available

Notes

Phenotypic Similarity to Human Syndrome: Anaplastic Large Cell Lymphoma J: 235890 in double Tg(Cd4-NPM/ALK)N1Ingh and Tg(TcraTcrb)1100Mjb mice.

References

• Original: J:92867 Hogquist KA, et al., T cell receptor antagonist peptides induce positive selection. Cell. 1994 Jan 14;76(1):17-27
• All: 1778 reference(s)