Prkci^tm1Hed
Targeted Allele Detail

Summary

• Symbol: Prkci^tm1Hed
• Name: protein kinase C, iota; targeted mutation 1, Stephen M Hedrick
• MGI ID: MGI:3054940
• Synonyms: PKClamda^-
• Gene: Prkci Location: Chr3:31049893-31106889 bp, + strand Genetic Position: Chr3, 14.65 cM
• Alliance: Prkci^tm1Hed page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:92719
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Exon 9 and intron 9, containing the ATP binding site and the splice acceptor site for exon 10, were replaced with a PGK-neobpA cassette in the opposite transcriptional orientation. RT-PCR from day 7.5 mutant embryos showed there was no transcript. (J:92719)

Expression

In Mice Carrying this Mutation:

1 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Prkci Mutation: 69 strains or lines available

References

• Original: J:92719 Soloff RS, et al., Targeted deletion of protein kinase C lambda reveals a distribution of functions between the two atypical protein kinase C isoforms. J Immunol. 2004 Sep 1;173(5):3250-60
• All: 2 reference(s)