Tg(CAG-EGFP)B5Nagy
Transgene Detail

Summary

• Symbol: Tg(CAG-EGFP)B5Nagy
• Name: transgene insertion B5, Andras Nagy
• MGI ID: MGI:3055103
• Synonyms: actin-GFP, B5/EGFP, beta-actin:GFP, Efgp, Tg(ACTB-EGFP)B5Nagy, Tg (GFPU) 5Nagy, Tg(GFPU)5Nagy, TgN(GFPU)5Nagy
• Transgene: Tg(CAG-EGFP)B5Nagy Location: unknown
• Alliance: Tg(CAG-EGFP)B5Nagy page

Transgene origin

• Germline Transmission: Earliest citation of germline transmission: J:92926
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Transgene description

• Transgene Type: Transgenic (Reporter)
• Mutation: Insertion
• Mutation details: The construct contains an enhanced green fluorescent protein gene driven by the CMV intermediate early enhancer coupled to the chicken beta actin promoter. The transgene integrated occurred on an unidentified autosome, and is expressed in all nucleated embryonic tissues. (J:92926)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available

Notes

In transgenic newborn and adult mice, the entire organ system expresses GFP. While transgene expression is ubiquitous, expression levels vary between organs. Cells and tissues with increased hemoglobin content exhibit reduced fluorescence as development progresses.

References

• Original: J:92926 Hadjantonakis AK, et al., Generating green fluorescent mice by germline transmission of green fluorescent ES cells. Mech Dev. 1998 Aug;76(1-2):79-90
• All: 95 reference(s)