Jam3^tm1Rha
Targeted Allele Detail

Summary

• Symbol: Jam3^tm1Rha
• Name: junction adhesion molecule 3; targeted mutation 1, Ralf H Adams
• MGI ID: MGI:3055281
• Synonyms: Jam-C-
• Gene: Jam3 Location: Chr9:27008680-27066717 bp, - strand Genetic Position: Chr9, 12.27 cM
• Alliance: Jam3^tm1Rha page

Small body size, severe hydrocephalus, and brain alterations in Jam3^tm1Rha/Jam3^tm1Rha mice

Show the 6 phenotype image(s) involving this allele.

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:92665
• Parent Cell Line: E14K (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: ES cells were transfected with a construct containing exons 4 and 5 flanked by loxP sites. The construct also contained an IRES-lacZ-pA cassette fused to the 5' end of exon 4 and a neomycin resistance cassette surrounded by Frt sites. This allele was generated by crossing mice carrying the floxed gene with mice carrying PGK-Cre and and a second strain of mice carrying the Flp transgene. Western blot analysis of E12.5 embryos did not detect protein. (J:92665)

Expression

In Structures Affected by this Mutation:

13 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Jam3 Mutation: 24 strains or lines available

References

• Original: J:92665 Gliki G, et al., Spermatid differentiation requires the assembly of a cell polarity complex downstream of junctional adhesion molecule-C. Nature. 2004 Sep 16;431(7006):320-4
• All: 10 reference(s)