Cacna2d2^tm1Svi
Targeted Allele Detail

Summary

• Symbol: Cacna2d2^tm1Svi
• Name: calcium channel, voltage-dependent, alpha 2/delta subunit 2; targeted mutation 1, Sergey V Ivanov
• MGI ID: MGI:3055290
• Synonyms: Cacna2d2^tm1Ncif
• Gene: Cacna2d2 Location: Chr9:107276948-107406545 bp, + strand Genetic Position: Chr9, 58.02 cM
• Alliance: Cacna2d2^tm1Svi page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:92396
• Parent Cell Line: CJ7 (ES Cell)
• Strain of Origin: 129S1/Sv-Oca2⁺ Tyr⁺ Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A neomycin resistance cassette replaced exons 33-39 of the gene. The replacement eliminated the delta subunit and the 87 C-terminal amino acids of the alpha-2 subunit of the protein. Northern blots of brain detected the truncated 5' mRNA and did not detect the 3' region. RT-PCR derived from brain did not detect the targeted 3' portion of the transcript. (J:92396)

Expression

In Structures Affected by this Mutation:

6 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Cacna2d2 Mutation: 72 strains or lines available

References

• Original: J:92396 Ivanov SV, et al., Cerebellar ataxia, seizures, premature death, and cardiac abnormalities in mice with targeted disruption of the Cacna2d2 gene. Am J Pathol. 2004 Sep;165(3):1007-18
• All: 2 reference(s)