Tnnt2^tm1Mmto
Targeted Allele Detail

Summary

• Symbol: Tnnt2^tm1Mmto
• Name: troponin T2, cardiac; targeted mutation 1, Sachio Morimoto
• MGI ID: MGI:3055296
• Synonyms: cTnT^-
• Gene: Tnnt2 Location: Chr1:135764092-135779998 bp, + strand Genetic Position: Chr1, 59.32 cM
• Alliance: Tnnt2^tm1Mmto page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:141967
• Parent Cell Line: CCE/EK.CCE (ES Cell)
• Strain of Origin: 129S/SvEv-Gpi1^c

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Insertion
• Mutation details: A floxed neo cassette was inserted between exons 12 and 13 and an exon 13 containing a deletion of three base pairs that results in the deletion of lysine at position 210 (K210) were inserted via homologous recombination. Immunohistochemical and western blot analysis indicate absence of protein expression in hearts from homozygous mice. (J:141967)

Phenotypes

Key:

hm	homozygous	ht	heterozygous	tg	involves transgenes	√	phenotype observed
cn	conditional genotype	cx	complex: > 1 genome feature	ot	other: hemizygous, indeterminate,...	N	normal phenotype

Genotype/
Background:

	Allelic Composition	Genetic Background	Cell Line(s)
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hm1	Tnnt2^tm1Mmto/Tnnt2^tm1Mmto	involves: 129S/SvEv
ht2	Tnnt2^tm1Mmto/Tnnt2^+	involves: 129S/SvEv

Phenotypes:

Affected Systems	hm1	ht2
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cardiovascular system	√
absent atrioventricular cushions	√
failure of heart looping	√
dilated heart	√
absent heartbeat	√
embryo	√
decreased embryo size	√
abnormal embryonic tissue morphology	√
growth/size/body	√
decreased embryo size	√
mortality/aging	√
embryonic lethality during organogenesis, complete penetrance	√
muscle	√
abnormal sarcomere morphology	√
normal phenotype		√
no abnormal phenotype detected		√

View phenotypes and curated references for all genotypes (concatenated display).

Disease models

Key:

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disease model

expected model not found

Models:

Human Diseases	ht2
dilated cardiomyopathy 1D IDs dilated cardiomyopathy 1D       DOID:0110426 ICD10CM:I42.0 OMIM:601494 Close

Expression

In Mice Carrying this Mutation:	1 assay results
In Structures Affected by this Mutation:	3 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Tnnt2 Mutation: 52 strains or lines available

References

• Original: J:141967 Nishii K, et al., Targeted disruption of the cardiac troponin T gene causes sarcomere disassembly and defects in heartbeat within the early mouse embryo. Dev Biol. 2008 Oct 1;322(1):65-73
• All: 4 reference(s)