Tnnt2^tm1Mmto
Targeted Allele Detail

Summary

• Symbol: Tnnt2^tm1Mmto
• Name: troponin T2, cardiac; targeted mutation 1, Sachio Morimoto
• MGI ID: MGI:3055296
• Synonyms: cTnT^-
• Gene: Tnnt2 Location: Chr1:135764092-135779998 bp, + strand Genetic Position: Chr1, 59.32 cM
• Alliance: Tnnt2^tm1Mmto page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:141967
• Parent Cell Line: CCE/EK.CCE (ES Cell)
• Strain of Origin: 129S/SvEv-Gpi1^c

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Insertion
• Mutation details: A floxed neo cassette was inserted between exons 12 and 13 and an exon 13 containing a deletion of three base pairs that results in the deletion of lysine at position 210 (K210) were inserted via homologous recombination. Immunohistochemical and western blot analysis indicate absence of protein expression in hearts from homozygous mice. (J:141967)

Disease models

Expression

In Mice Carrying this Mutation:	1 assay results
In Structures Affected by this Mutation:	3 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Tnnt2 Mutation: 51 strains or lines available

References

• Original: J:141967 Nishii K, et al., Targeted disruption of the cardiac troponin T gene causes sarcomere disassembly and defects in heartbeat within the early mouse embryo. Dev Biol. 2008 Oct 1;322(1):65-73
• All: 4 reference(s)