Myf5^tm1Tajb
Targeted Allele Detail

Summary

• Symbol: Myf5^tm1Tajb
• Name: myogenic factor 5; targeted mutation 1, Shahragim Tajbakhsh
• MGI ID: MGI:3055340
• Synonyms: Myf5^GFP-P
• Gene: Myf5 Location: Chr10:107318769-107321995 bp, - strand Genetic Position: Chr10, 55.95 cM
• Alliance: Myf5^tm1Tajb page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:92799
• Parent Cell Line: CK35 (ES Cell)
• Strain of Origin: 129S2/SvPas

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A targeting vector was designed to insert a cassette containing EGFP-pA-loxP-puro-loxP into exon 1, thereby deleting the 122 amino acids encoding the basic helix-loop-helix domain. Additionally, the two ATG codons were mutated to GTG. The EGFP contains a Val164Ala mutation in pEGFP-C1. (J:92799)

Expression

In Mice Carrying this Mutation:	23 assay results
In Structures Affected by this Mutation:	3 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Myf5 Mutation: 17 strains or lines available

References

• Original: J:92799 Kassar-Duchossoy L, et al., Mrf4 determines skeletal muscle identity in Myf5:Myod double-mutant mice. Nature. 2004 Sep 23;431(7007):466-71
• All: 11 reference(s)