Myf5^tm2Tajb
Targeted Allele Detail

Summary

• Symbol: Myf5^tm2Tajb
• Name: myogenic factor 5; targeted mutation 2, Shahragim Tajbakhsh
• MGI ID: MGI:3055343
• Synonyms: Myf5^loxP
• Gene: Myf5 Location: Chr10:107318769-107321995 bp, - strand Genetic Position: Chr10, 55.95 cM
• Alliance: Myf5^tm2Tajb page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:92799
• Parent Cell Line: CK35 (ES Cell)
• Strain of Origin: 129S2/SvPas

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A targeting vector was designed to delete the amino-terminal domain following cre-mediated recombination, leaving a single loxP site upstream of the basic helix-loop-helix domain. The endogenous ATG was mutated to GTG, leaving the first ATG codons suitable for translation located in the chromatin remodeling and basic domains, at residues 69 and 82, respectively. This alleles was generated from Myf5^tm3Tajb. (J:92799)

Expression

In Mice Carrying this Mutation:	10 assay results
In Structures Affected by this Mutation:	4 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Myf5 Mutation: 17 strains or lines available

References

• Original: J:92799 Kassar-Duchossoy L, et al., Mrf4 determines skeletal muscle identity in Myf5:Myod double-mutant mice. Nature. 2004 Sep 23;431(7007):466-71
• All: 12 reference(s)