F9^tm2(F9)Dws
Targeted Allele Detail

Summary

• Symbol: F9^tm2(F9)Dws
• Name: coagulation factor IX; targeted mutation 2, Darrel W Stafford
• MGI ID: MGI:3055509
• Synonyms: R333Q-hFIX
• Gene: F9 Location: ChrX:59044824-59076119 bp, + strand Genetic Position: ChrX, 33.5 cM, cytoband A6-A7
• Alliance: F9^tm2(F9)Dws page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:92964
• Parent Cell Line: TC1/TC-1 (ES Cell)
• Strain of Origin: 129S6/SvEvTac

Mutation description

• Allele Type: Targeted
• Mutation: Insertion
• Mutation details: The targeting construct includes the signal sequence, first intron and propeptide of the mouse gene, the equivalent human cDNA and a neomycin cassette. Two site specific point mutations, T148A and R333Q were created in the human cDNA. The human cDNA is joined to the mouse gene at codons 6 and 7, such that the mouse expresses a missense mutant human protein, but does not express the mouse protein. ELISA analysis using specific human anti-F9 polyclonal antibodies detects human F9 in homozygous mice. (J:92964)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any F9 Mutation: 8 strains or lines available

References

• Original: J:92964 Jin DY, et al., Creation of a mouse expressing defective human factor IX. Blood. 2004 Sep 15;104(6):1733-9
• All: 1 reference(s)